Bioinformatics Questions

Describe how individuals with one (heterozygous) or two (homozygous) copies of the wild-type GA3H allele can have the same phenotype.

Assuming that species I is the ancestral species of the group, explain the most likely genetic change that produced the polypeptide in species II and the most likely genetic change that produced the polypeptide in species III.

Epigenetics is the study of heritable changes in the phenotype caused by mechanisms other than changes in the DNA sequence. Describe ONE example of epigenetic inheritance.

The wild-type allele encodes a GA3H enzyme with alanine (Ala), a nonpolar amino acid, at position 229. The mutant allele encodes a GA3H enzyme with threonine (Thr), a polar amino acid, at position 229. Describe the effect of the mutation on the enzyme and provide reasoning to support how this mutation results in a short plant phenotype in homozygous recessive plants.

The primary transcript in the figure is 15 kilobases (kb) long, but the mature mRNA is 7kb in length. Describe the modification that most likely resulted in the 8 kb difference in length of the mature mRNA molecule. Identify in your response the location in the cell where the change occurs.

Yeast mate by fusing two haploid cells to make a diploid cell. In a second experiment, the researcher mates the Mutant 1 and Mutant 2 haploid strains to produce diploid cells. Using the table provided, predict whether the diploid cells will grow on each of the four media. Use a plus sign (+) to indicate growth and a minus sign (−) to indicate no growth

Using the codon chart provided, predict the change in the codon sequence that resulted in the substitution of alanine for threonine at amino acid position 229.

The researcher observed that tissues with a high level of gene H mRNA did not always have gene H protein. Provide reasoning to explain how tissues with high gene H mRNA levels can have no gene H protein

Provide ONE piece of evidence that would indicate new genetic variation has occurred in the engineered flies.

Describe ONE mechanism that could lead to genetic variation in the engineered strain of flies.

Predict the length of the mature gene X mRNA if the full-length gene is introduced and expressed in prokaryotic cells. Justify your prediction.

Based on the data provided, identify the gene that is most likely to encode a protein that is an essential component of glycolysis. Provide reasoning to support your identification.

Translation is the process of turning instructions from mRNA into chains of amino acids. It occurs in the cytoplasm with the help of ribosomes and tRNA.

DNA sequence provides the code for the amino acid sequence. The amino acid sequence determines the structure of the protein, which affects the function of the protein

In pBluescript II, MCS is part of the ______ gene

a sh_____ vector is a cloning vector capable of replicating in two or more organisms.

A researcher interested in the control of the cell cycle identifies a yeast mutant (cd-) whose rate of cell division is temperature sensitive. At low, permissive temperatures, the mutant strain grows normally and produces yeast colonies having a normal size. However, at elevated, restrictive temperatures, the mutant strain is unable to divide and produces no colonies. She has a yeast genomic library made in a plasmid vector (containing the ura3+ selectable marker) and a ura3- cd- mutant, and she wants to clone the gene affected in the cell division mutant.What steps should she take to accomplish this objective?

Which of the following is NOT included in a PCR reaction mix?a.) Double-stranded DNA templateb.) Primersc.) DNA Polymerased.) ddNTPse.) they are all included

The chemical label that permits visualization of DNA fragments in each lane of an automated sequencing isa.) ³²Pb.) ³²Sc.) a fluorescent chemicald.) tritium

Bacteria protect their own DNA from restriction enzyme damage by adding _____ groups to certain nucleotidesa.) methylb.) hydroxylc.) phosphated.) amino