Bioinformatics Questions

The guessmer approach is appropriate when you havea.) a partial peptide sequenceb.) a gene from a related speciesc.) an expression vectord.) a mutant

DNA is stained with ______, which binds to DNA and fluoresces under UV light.a.) NaOHb.) HNO₂c.) NH₂OHd.) EtBr

Which of the following can result from the insertion of a transposable element?a.) no effect on a nearby geneb.) production of a mutation by insertional mutagenesisc.) an increase or decrease in transcription activity of a nearby gened.) all of the above

The restriction enzyme HaeII was isolated froma.) Bacillus globigib.) Esherichia colic.) Haemophilus aegypticusd.) Haemophilus harmolyticus

Which of the following most often utilizes an expression vector?a.) PCRb.) The antibody-screening approach to find a specific clone in a libraryc.) Heterologous probingd.) Southern blotting

How many primers are used in a sequencing reaction and in a PCR reaction?a.) Sequencing = 1, PCR = 1b.) Sequencing = 1, PCR = 2c.) Sequencing = 2, PCR = 1d.) Sequencing = 2, PCR = 2

Two host enzymes, DNA pol_______ and ligase, are used to fill in the single stranded gaps created after the target sit cut and IS insertion.

In a transformation experiment involving pBSII, two chemicals are added to the agar medium for selection purposes. Name one of the chemicals.

Dissociation element (Ds) isa.) an activator transposon that can direct its own transpositionb.) lacking both transposases and IRsc.) involved in an unstable mutation that leads to a spotted kernel phenotyped.) a gene encoding the red anthocyanin pigment

In class, we learned about using a shuttle vector to identify genes by complementation. In this particular case, the shuttle vector has the ability toa.) move genes from the human yeastb.) replicate in yeast or in bacteriac.) degrade selected yeast genesd.) recombine with yeast nuclear genese.) degrade other yeast plasmids

Genomics is the sequencing, gene prediction, and functional analysis of the entire genome of an organism. It is often followed by:tr______omics (whole study of gene expression) and p____omics (global study of protein expression and function)

What is EST?a.) Extraterrestrial search toolb.) expressed sequence tag (partial cDNA sequence)c.) Euchromatin silencing testd.) Epigenetic sequence mark from transgene

the following is an example ofa.) a composite transposonb.) a noncomposite transposonc.) an insertion sequenced.) a retroelement

A microtiter plate from a genomic library contains frozena.) cDNAb.) RNAc.) bacteriad.) proteins

In theory, which of the following restriction sites should appear most frequently in a genome?a.) Sau3AI (GATC)b.) BAMHI (GGATCC)c.) NotI(GCGGCCGC)d.) their appearances should be equally frequent due to randomness

In order for the dissociation element (Ds) mutations in corn to be stable,a.) an Ac element must be presentb.) an Ac element must not be presentc.) the Ds must contain the gene for transpositiond.) the DNA must not be replicated

Bill wanted to insert a DNA fragment into the pBII vector using the blue/white selection protocol. Unfortunately, he forgot to add ampicillin and X-gal to the agar medium. What would be the outcome of the transformation experiment?a.) All white coloniesb.) All blue coloniesc.) Some white and some blue coloniesd.) No colonies

Which of the following elements are found in eukaryotes but not prokaryotes?a.) IS elementb.) Ac/Dsc.) LINE/SINEd.) Ty Elementse.) b, c, and d

Which of the following techniques discussed in class involves partial digestion?a.) Genomic library constructionb.) cDNA library constructionc.) All of the aboved.) None of the above

Which tRNA initiates translation? which amino acid does it deliver?