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Laboratory Safety: Biological Hazards and Containment Study Guide

This guide covers essential laboratory safety practices, hazard identification, and the regulatory framework for transporting dangerous goods. Learn about different types of hazards, risk mitigation strategies, and specific packaging requirements for infectious substances and other hazardous materials. Essential for anyone working in or managing laboratory environments.

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Safety and Emergency Procedures

Safety and Emergency Procedures in the Laboratory

Laboratory personnel must follow good laboratory practice, participate in risk assessments, and understand various biological, chemical, physical, and equipment hazards to minimize risks.

  • Hazard vs Risk
  • Hierarchy of Control
  • Engineering Controls
  • Administrative Controls
  • Personal Protective Equipment (PPE)
  • Risk Assessment
  • Workplace Hazardous Material Information System (WHMIS)
  • Safety Data Sheet (SDS)
  • Transportation of Dangerous Goods (TDG)
  • Biological Hazards
  • Chemical Hazards
  • Physical Hazards
  • Ergonomic Hazards
  • Psychological Hazards
  • Hand Hygiene
  • Decontamination, Disinfection, and Sterilization
  • Occupational Health and Safety Management Systems (OHSMS)
  • Routes of Entry
  • Laboratory Waste Disposal

Safety and Emergency Procedures for PPE

Procedures for hand hygiene and the proper use of personal protective equipment including gowns, gloves, eye protection, masks, respirators, and footwear.

  • Hand hygiene
  • Alcohol-based hand rubs
  • Personal protective equipment
  • Glove types and selection
  • Contact dermatitis
  • Safety goggles and face shields
  • Surgical masks
  • Laboratory footwear

Biological Hazards and Containment Procedures

This text outlines biological hazards, risk groups, containment levels, and the use of biological safety cabinets to protect against infectious agents.

  • Biological hazards
  • Human Pathogens and Toxins Act (HPTA)
  • Pathogen risk groups
  • Containment levels
  • Biological safety cabinets (BSC)
  • HEPA filtration
  • Class I, II, and III BSCs

Biological, Chemical, and Laboratory Waste Management

Procedures for managing biological spills, chemical hazards, and the proper use of biological safety cabinets.

  • Biological safety cabinet use
  • Biological spill response
  • Leaking specimen processing
  • Chemical hazard identification
  • Chemical storage and disposal
  • Chemical spill response
  • Personal protective equipment
  • Workplace Hazardous Materials Information System (WHMIS)

WHMIS 2015 and Laboratory Safety Procedures

This document outlines safety and emergency procedures including chemical spill response, WHMIS 2015 hazard classification, safety data sheets, and the proper use of chemical fume hoods and compressed gases.

  • chemical spill response
  • WHMIS 2015
  • Globally Harmonized System (GHS)
  • hazard classification
  • safety data sheets (SDS)
  • pictograms
  • signal words
  • hazard statements
  • precautionary statements
  • supplier labels
  • workplace labels
  • chemical fume hoods
  • laminar flow hoods
  • compressed gases
  • cryogenic hazards

Laboratory Hazards and Transportation of Dangerous Goods

This document outlines laboratory safety protocols regarding chemical, physical, and biological hazards, ergonomics, and the transportation of dangerous goods.

  • Dry ice hazards
  • Liquid nitrogen safety
  • Heat hazards
  • Electrical hazards
  • Ionizing radiation
  • Non-ionizing radiation
  • Ultraviolet light
  • Microwave risks
  • Noise exposure
  • Pressure differences
  • Ergonomics
  • Repetitive stress injuries
  • Psychological hazards
  • Reproductive hazards
  • Transportation of Dangerous Goods (TDG)
  • Infectious substances
  • Category A and B substances
  • Medical or clinical waste
  • Packaging requirements

Fire Safety and Emergency Response Protocols

Procedures for managing injuries, centrifuge accidents, fire safety, and various workplace emergencies.

  • Needlestick and blood exposure procedures
  • Centrifuge breakage protocols
  • Fire safety basics
  • Flammable and combustible material definitions
  • Fire triangle components
  • Classes of fire
  • Types of fire extinguishers
  • PASS acronym for extinguisher use
  • Emergency evacuation plans
  • First aid requirements
  • Safety Data Sheets (SDS)

Decontamination Procedures and Emergency Responses

Safety and Emergency Procedures

Procedures for decontamination, disinfection, and sterilization of medical equipment and laboratory materials, alongside protocols for handling dangerous goods and emergency responses.

  • Decontamination
  • Disinfection
  • Sterilization
  • Sanitation
  • Cleaning
  • Autoclave
  • Ethylene oxide
  • Incineration
  • Spaulding classification
  • Critical items
  • Semi-critical items
  • Non-critical items
  • Biological indicators
  • Chemical indicators
  • Alcohols
  • Chlorine and Chlorine Compounds
  • Hydrogen Peroxide
  • Peracetic acid
  • Iodophors
  • Phenolics

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