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Read the passage and answer the question."Your picture may be very exact, Louisa," said Bingley; "but this was all lost upon me. I thought Miss Elizabeth Bennet looked remarkably well when she came into the room this morning. Her dirty petticoat quite escaped my notice.""YOU observed it, Mr. Darcy, I am sure," said Miss Bingley; "and I am inclined to think that you would not wish to see YOUR sister make such an exhibition." "Certainly not.""To walk three miles, or four miles, or five miles, or whatever it is, above her ankles in dirt, and alone, quite alone! What could she mean by it? It seems to me to show an abominable sort of conceited independence, a most country-town indifference to decorum."What do the underlined words in this reading passage most likely mean?
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- *10.19 A 10-kb genomic DNA EcoRI fragment from a newly discovered insect is ligated into the EcoRI site of the pBluescript II plasmid vector (described in Figure8.4, p. 176) and transformed into E. coli. Plasmid DNA and genomic DNA from the insect are prepared and each DNA sample is digested completely with the restrictionenzyme EcoRI. The two digests are loaded into separate wells of an agarose gel, and electrophoresis is used to separate the products by size.a. What will be seen in the lanes of the gel after it is stained to visualize the size-separated DNA molecules?b. What will be seen if the gel is transferred to a membraneto make a Southern blot, and the blot is probed with the 10-kb EcoRI fragment? (Assume the fragment does not contain any repetitive DNA sequence.)
- استلَفَ = استدانْت خمسين جنيها من فاطمةاستعرت سيارة جارتي لاذهب إلى المستشفى ، استعرت كتابا من المكتبةاقترض الرجل 50000 دولار من البنك ، وإذا لم يرد القَرض ستقبض عليه الشرطة ، اقترضت سيارة جارتياستلفت خمسين جنيها من فاطمة ، استلفت سيارة فاطمة