Biotechnology Questions

animals engineered to serve as models to help and potentially treat human diseases

over __ of the nations production of corn, cotton, and soybeans comes from genetically modified crops

how much processed food contains GMOS

genetically engineered (GE) crops and animals are regulated by

What are competent cells treated with in order to become competent

What prevents formation of ice crystals inside the cells

Why are the cells kept in nutrient broth for a short period during transformation

What process was used to separate components

What needs to be binded to AraC protein in order to switch on gfp production

If arabinose is added to a media will the transformed cells be able to produce GFP?

What are the repeating subunits called that make up DNA?

How many genes does the human genome consist of? (roughly)

T or F A gene can code for the same polypeptide whether it is a human cell, bacterium or any other cell

How many copies of DNA sequences would 30 cycles yield?

T or F Smaller base pairs move further than larger base pairs

Do the amount of satellite DNA (STR) repeats vary greatly between different individuals?

What are organisms that acquire a foreign gene called?

What is the term to describe how at restriction sites, the sequence of one strand is identical to the complementary sequence on the other strand when both are read from 5' to 3' direction

Diabetes is a cluster of metabolic disorders where individuals suffer from elevated blood sugar levels. To regulate their blood sugar, some diabetics must inject themselves daily with the hormone insulin, which reduces the amount of sugar in the bloodstream. Historically, therapeutic insulin has been produced by pigs and other animals. However, raising pigs and purifying their insulin for medical use is labor intensive and pig-derived insulin can produce immune reactions in some people. Based on the techniques of biotechnology, what would be the best alternative for producing therapeutic insulin?

Polymerase chain reaction (PCR) is a powerful tool in molecular biology that is used to amplify a particular DNA sequence. If a PCR reaction initially contains 1 double-stranded DNA copy of the sequence of interest, how many copies of double-stranded DNA will be generated after 12 cycles? Assume perfect doubling occurs in each cycle.