Video Penjelasan Isolasi PGPR dari Tanaman Putri Malu

Mari kita pelajari proses persiapan sampel dan alat untuk isolasi PGPR dari akar putri malu.Langkah pertama adalah pengambilan sampel akar putri malu yang sehat dari tanah.Akar dibersihkan dengan air mengalir untuk menghilangkan tanah yang menempel.Selanjutnya, dilakukan sterilisasi permukaan akar menggunakan dua larutan.Pertama, akar direndam dalam alkohol 70% selama 3 menit untuk sterilisasi awal.Kemudian dipindahkan ke larutan sodium hipoklorit 2% selama 2 menit untuk sterilisasi lanjutan.Sementara itu, kita persiapkan alat-alat yang diperlukan untuk proses isolasi.Alat-alat yang dibutuhkan meliputi cawan petri, tabung reaksi, media NA atau Nutrient Agar, dan larutan garam fisiologis.Semua peralatan ini harus dalam kondisi steril untuk menghindari kontaminasi.The sterilized roots are cut into small segments of approximately 1 centimeter.These segments are then placed in physiological saline solution for bacterial extraction.A series of dilutions is performed, from ten to the negative one through ten to the negative seven, to obtain isolated colonies.Using the spread plate method, zero point one milliliter of each dilution is spread onto nutrient agar plates.The plates are then incubated at room temperature for twenty-four to forty-eight hours.After incubation, bacterial colonies with different characteristics will appear on the plates.Distinct colonies are then transferred to fresh nutrient agar media for purification.Let's examine the morphological characteristics of our isolated bacterial colonies.We observe various colony characteristics including shape, color, elevation, and margin type.Next, we perform Gram staining to classify our bacteria.The staining results show both Gram-positive bacteria appearing purple, and Gram-negative bacteria appearing pink.Various biochemical tests are performed to identify metabolic characteristics of our isolates.Finally, we assess PGPR characteristics that make these bacteria beneficial for plant growth.In conclusion, we have successfully identified and characterized our PGPR isolates through multiple testing methods.These bacteria can now be developed further as biofertilizers.