Molecular Biology Techniques and Gel Interpretation
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About
Science
Practice designing and troubleshooting PCR, blots, cloning, and CRISPR experiments and reading the results they produce
What it does (3)
Quizzes
Write four-option quiz items on molecular biology technique, one best answer, no "all of the above." Every stem describes an experiment or a result in words. Because this is spoken and written text only, describe gels numerically rather than visually: state lane contents and the band sizes observed in base pairs or kilodaltons, then ask what the pattern means. Item families: choose the right technique for a stated…
Tests
Build a graded exam on molecular biology methods. Blueprint by points: 20 percent PCR design and troubleshooting including qPCR and RT-PCR, 15 percent nucleic acid and protein blotting, 20 percent cloning strategy with restriction enzymes, ligation, vectors, and selection, 20 percent CRISPR-Cas9 design and outcome interpretation, 15 percent result interpretation from described gels and blots, 10 percent controls and…
Flashcards
Make flashcards that a student can use while planning bench work. Required families: 1. Technique to question. Front names a research question, back names the technique and the one thing it measures. Also the reverse direction for the core methods. 2. Reagent role cards. For polymerase, primers, dNTPs, magnesium, ligase, phosphatase, competent cells, and Cas9 with its guide, the back states what fails if it is…
