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MÉTHODE DE SANGER Objectif: Déterminer la succession de _________ composant un fragment d'ADN***Outils nécessaires:-__________*-Taq polymérase -______________***dNTP en escès-Didésoxynucléotides ddNTP***(et autres) -En faible quantité -Sans OH sur les carbones __' et __' du PENTOSE*** -Pour la terminaison de la chaîne en cours de synthèse*Principe de la méthode: réaction d'élongation effectuée en __________, avec les 4 ddNTP pour un même fragment d'ADN
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