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Below are the steps of a Western blot. What is the correct order?1. The membrane is incubated with the secondary antibody.2. The membrane is incubated with the primary antibody.3. The membrane is washed with a blocking solution.4. Proteins are transferred to a nitrocellulose membrane. 5. Protein extracts are separated by gel electrophoresis.a.5, 4, 3, 2, 1b.4, 5, 2, 3, 1c.3, 4, 5, 1, 2d.2, 5, 4, 3, 1
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