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*10.12 A colleague has sent you a 4,500-bp DNA fragment excised from a plasmid cloning vector with the enzymes PstI and BglII (see Table 8.1, p. 174, for a descriptionof these enzymes and the sites they recognize). Your colleague tells you that within the fragment there is an EcoRI site that lies 490 bp from the PstI site.a. List the steps you would take to clone the PstI-BglIIDNA fragment into the plasmid vector pBluescript II (described in Figure 8.4, p. 176).b. How would you verify that you have cloned the correct fragment and how would you determine its orientation within the pBluescript II cloning vector?
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